OVA/B16-F10
CBPG0012
詢(xún) 價(jià)
留 言
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫(kù)
| I. Background | |
| OVA 基因（Ovalbumin）是雞卵清蛋白編碼基因，分子量約 43kDa，含 12269 個(gè)氨基酸，為免疫學(xué)與腫瘤研究的核心工具。其表位如 SIINFEKL 可激活 CD8+ T 細(xì)胞，用于構(gòu)建穩(wěn)轉(zhuǎn)細(xì)胞系評(píng)估 CAR-T/TCR-T 療效及疫苗免疫應(yīng)答。OVA 四聚體技術(shù)可精準(zhǔn)檢測(cè)抗原特異性 T 細(xì)胞，在腫瘤免疫監(jiān)測(cè)、表觀遺傳調(diào)控（如 DNA 甲基化）及胚胎發(fā)育研究中發(fā)揮關(guān)鍵作用，是精準(zhǔn)免疫醫(yī)學(xué)的重要實(shí)驗(yàn)平臺(tái)。 | |
| II. Introduction | |
| Host Cell: | B16-F10 |
| Expressed gene: | OVA |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | skin |
| Morphology: | mixture of spindle-shaped and epithelial-like cells |
| Growth Properties: | Adherent |
| IV. Representative Data | |
|
Figure 1. WB of OVA/B16-F10. |
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| V. In VIVO Data | |
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Index |
Content |
|
Animal |
C57BL/6J, 7weeks, male |
|
Implanted Position |
Subcutaneous (right lower flank region ) |
|
Injected Cell Numbers |
2×10^5/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. OVA/B16-F10 Mean Body Weight ± SEM.
2. Tumor Volume
Figure 3. OVA/B16-F10 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
