Mouse NK2R HEK293
CBP71645
詢 價
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產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| I. Background | |
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小鼠 NK2R（Neurokinin-2 Receptor），基因正式名稱為 Tacr2（也稱 NK2R / TAC2R / SKR），編碼速激肽受體家族的 G 蛋白偶聯(lián)受體，特異性結(jié)合內(nèi)源性配體神經(jīng)激肽 A（Neurokinin A / Substance K），親和順序為 NKA > NKB > SP。 它在小鼠中樞（下丘腦、邊緣系統(tǒng)）及外周組織（胃腸道、氣道、子宮平滑肌等）廣泛表達，通過 Gαq?PLC?IP??Ca²? 通路介導(dǎo)平滑肌收縮、神經(jīng)遞質(zhì)釋放、痛覺傳遞及生殖軸 LH 分泌調(diào)節(jié)；近年研究還發(fā)現(xiàn)下丘腦 NK2R 激活可抑制食欲、增加能量消耗，是肥胖與糖代謝調(diào)控的潛在靶點。 |
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| II. Description | |
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Mouse NK2R HEK293 細胞，在 HEK293 細胞的基礎(chǔ)上，穩(wěn)定表達Mouse NK2R。 |
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| III. Introduction | |
| Expressed gene: | Mouse NK2R |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM + 10%FBS + 2μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional assay for Mouse NK2R |
| Transducer: | Gq/Gs |
IV. Representative Data Figure 1. qPCR of Mouse NK2R HEK293.  Figure 2. HTRF cAMP Assay with Mouse NK2R HEK293 (C12) in 500 μM IBMX.  Figure 3. HTRF IP-One Assay with Mouse NK2R HEK293(C12). |
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