32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:
90% FBS+10% DMSO
Culture Medium:
DMEM+10%FBS+2μg/ml Puromycin
Mycoplasma Status:
Negative
Storage:
Liquid nitrogen
II. Description of Host Cell Line
Organism:
Homo sapiens,human
Tissue:
Mammary gland breast;derived from metastatic site:pleural effusion
Disease:
Adenocarcinoma
Morphology:
Epithelial
Growth Properties:
Adherent
III. Representative Data
Figure 1.Detect Luciferase assay byUltra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). The number of Luminescence signal is 551±36(Mean±SD) on MDA-MB-231 cells while 18434541±482490(Mean±SD) on MDA-MB-231-Luc cells.
IV. Stablity Test
Figure 2. Stability test results. MDA-MB-231-Luc cells showed at least 32 passage stability with similar luminescence unit.
V. In VIVO Data
Index
Content
Animal
BALB/C Nude, 7-8weeks, Female
Implanted Position
i.p.
Injected Cell Numbers
1×10^7/0.2mL
Matrix gel
Without matrix gel
1. Weight Change
Figure 3. MDA-MB-231-Luc Mean Body Weight ± SEM.
2. Total Flux
Figure 4. Total flux MDA-MB-231-Luc .
NOTE:The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).