32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:
90% FBS+10% DMSO
Culture Medium:
RPMI-1640+10%FBS+1ug/ml puromycin
Mycoplasma Status:
Negative
Storage:
Liquid nitrogen
II. Description of Host Cell Line
Organism:
Homo sapiens,human
Tissue:
Bone marrow
Disease:
Plasmacytoma;myeloma
Morphology:
Lymphoblast
Growth Properties:
Suspension
III. Representative Data
Figure. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System..
The number of Luminescence signal is 503±12(Mean±SD) on NCI-H929 cells while 6003309±123057(Mean±SD) on NCI-H929-Luc cells.
IV. Stablity Test
Figure. Stability test results. NCI-H929-Luc cells showed at least 32 passage stability with similar luminescence unit.