hGM-CSF Effector Reporter Cell
CBP74149
| I. Background | |
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粒細胞-巨噬細胞集落刺激因子（GM-CSF）是一種多功能的造血生長因子和免疫調(diào)節(jié)劑，最初因發(fā)現(xiàn)其能刺激骨髓祖細胞增殖分化為粒細胞和巨噬細胞而得名。它可由多種細胞類型產(chǎn)生，包括活化的 T 細胞、巨噬細胞、成纖維細胞、內(nèi)皮細胞等，通常在感染、炎癥或組織損傷等應(yīng)激狀態(tài)下被大量分泌。GM-CSF 不僅參與維持造血穩(wěn)態(tài)，更是連接先天性免疫和適應(yīng)性免疫的關(guān)鍵橋梁，在炎癥反應(yīng)、自身免疫疾?。ㄈ珙愶L(fēng)濕性關(guān)節(jié)炎）和組織修復(fù)中發(fā)揮核心作用。 |
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| II. Description | |
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GM-CSF Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi) GM-CSF 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
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| III.. Introduction | |
| Expressed gene: |
GMRα&GMRβ
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| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10% FBS+100μg/ml Hygromycin B+2 ng/ml GM-CSF |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
Figure 2. Dose Response of Recombinant Human GM-CSF in GM-CSF Effector Reporter Cell (C10).
Figure 3. Inhibition of hGM-CSF-induced Reporter Activity by hGM-CSF Neutralizing Antibody in hGM-CSF Effector Reporter Cell (C10).
