I. Background
腫瘤壞死因子-α（TNF-α）是一種分子量為26 kDa 的跨膜蛋白，其前肽在TNF-α-轉(zhuǎn)換酶（TACE）裂解時(shí)以可溶性17 kDa 分子的形式分泌。雖然活化的巨噬細(xì)胞是TNF-α的主 要來(lái)源，但它也可以由多種其他細(xì)胞產(chǎn)生，如成纖維細(xì)胞、星形膠質(zhì)細(xì)胞、平滑肌細(xì)胞、 角化細(xì)胞和多種腫瘤細(xì)胞，包括B 細(xì)胞淋巴瘤、乳腺癌和結(jié)腸癌。       TNF-α作為腫瘤促進(jìn)因子，參與腫瘤發(fā)生的所有步驟，包括腫瘤的轉(zhuǎn)化、增殖、血管生成、侵襲和轉(zhuǎn)移。TNF α信號(hào)傳導(dǎo)也是免疫系統(tǒng)的重要組成部分，可抑制腫瘤發(fā)生，阻止病毒復(fù)制，是一種內(nèi)源性熱原，可誘導(dǎo)發(fā)燒和細(xì)胞凋亡。
II. Description
Membrane Anchored TNFαCell(Adherent)作為ADCC Bioassay Effector Cell V variant (High Affinity)-Fcγ-NFAT Jurkat 或TNFR2(TNFR1 KO)Effector Reporter Cell 的靶細(xì)胞，很好的模擬了體內(nèi)ADCC 效應(yīng)（原理見(jiàn)Figure 1所示）或TNFR2 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程（原理見(jiàn)Figure 2所示）。 Figure 1. ADCC 效應(yīng)的細(xì)胞模型原理圖 Figure 2. TNFR2 信號(hào)轉(zhuǎn)導(dǎo)的細(xì)胞模型原理圖
III. Introduction
Expressed gene:	TNFα
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	F12K+10%FBS+5μg/ml Puromycin
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay
IV. Representative Data

Figure 3. Recombinant Membrane Anchored TNFα Cell (Adherent) constitutively expressing TNFα.

Figure 4. Dose Response of TNFα Abs in ADCC Bioassay Effector Cell V variant (High Affinity)-Fcγ-NFAT-Jurkat (C36) with Membrane Anchored TNFα Cell (Adherent, C12).



Figure 5. Blocking of TNFα induced TNFR2( TNFR1 KO) Effector Reporter Cell(C64) Activity by TNFα Neutralization Abs with Membrane Anchored TNFα Cell(Adherent, C12).


Figure 6. Blocking of TNFα induced TNFR2(TNFR1 KO) Effector Reporter Cell( C64) Activity by TNFR2 Blocking Ab with Membrane Anchored TNFα Cell ( Adherent, C12).